The times of birth of Arthur and his sister, Margaret (see her chart), were recorded in the Beaufort Book of Hours, a family chronicle inherited by Henry VIII’s grandmother, Lady Margaret Beaufort. While the date of birth of the future Henry VIII was recorded in the book, his time of birth was not recorded, because he was the third born of Henry VII's children and thus he did not rank highly enough.
According to the Book, "in the morning afore one of the clock after midnight was born Prince Arthur at Winchester."
Read more from his Wikipedia biography.
NATAL DATA and ASTROLOGY CHART
BIRTH DATA: 20 September 1486, about 00:50 (12:50 a.m.) LAT (-0:04:22), Winchester, England (51n04, 1w19). ASC: about 9 Leo. RR: AA (from family chronicle). SOURCE: Sy Scholfield (©) quotes The Beaufort Book of Hours, a chronicle inherited by Henry VIII’s grandmother, Lady Margaret Beaufort, which states that on 20 September 1486 (Julian Calendar) "in the morning afore one of the clock after midnight was born Prince Arthur at Winchester." http://www.bl.uk/onlinegallery/onlineex/henryviii/birthaccdeath/beaufort.
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